gchapman5593 gchapman5593
  • 20-12-2017
  • Biology
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How does crispr target the gene of interest?

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Diatonic254
Diatonic254 Diatonic254
  • 23-12-2017

CRISPR is mechanism tapped and modified from bacterial defense mechanism to form a tool to edit DNA in a genome. CRISPR is composed of palindromic sequences interspersed with foreign spacer DNA. The spacer DNA is transcribed into crRNA that is guided by a tracrRNA to the invading helical foreign DNA. The tracrRNA recruits Cas9 protein which helps cut the exogenous DNA.





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